| 1. Round | 10/08/2024 | Tranmere Rovers | - | Notts County | 0:0 | ||||
| 2. Round | 17/08/2024 | Port Vale FC | - | Tranmere Rovers | 0:0 | ||||
| 6. Round | 14/09/2024 | Gillingham FC | - | Tranmere Rovers | 3:0 | ||||
| 7. Round | 21/09/2024 | Colchester United | - | Tranmere Rovers | 3:0 | ||||
| 8. Round | 27/09/2024 | Tranmere Rovers | - | Salford City | 0:0 |  |
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| 12. Round | 19/10/2024 | Bromley FC | - | Tranmere Rovers | 1:2 | |
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| 13. Round | 22/10/2024 | Tranmere Rovers | - | Grimsby Town | 0:1 | |
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| 14. Round | 26/10/2024 | Crewe Alexandra | - | Tranmere Rovers | 3:1 | ||||
| 15. Round | 09/11/2024 | Tranmere Rovers | - | Newport County | 2:1 | ||||
| 17. Round | 22/11/2024 | Cheltenham Town | - | Tranmere Rovers | 1:0 | |
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| 31. Round | 08/02/2025 | Salford City | - | Tranmere Rovers | 2:0 | ||||
| 16. Round | 11/02/2025 | Tranmere Rovers | - | Fleetwood Town | 0:0 | ||||
| 32. Round | 15/02/2025 | Tranmere Rovers | - | Milton Keynes Dons | 1:1 |  |
England