| 3. Round | 07/08/2021 | Dynamo České Budějovice | - | Baník Ostrava | 1:3 | ||||
| 4. Round | 14/08/2021 | Sigma Olomouc | - | Dynamo České Budějovice | 3:3 |  |
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| 5. Round | 22/08/2021 | Dynamo České Budějovice | - | Slovan Liberec | 1:0 | ||||
| 6. Round | 28/08/2021 | AC Sparta Praha | - | Dynamo České Budějovice | 1:0 | |
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| 7. Round | 12/09/2021 | Dynamo České Budějovice | - | FC Hradec Králové | 0:1 | |
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| 8. Round | 18/09/2021 | Viktoria Plzeň | - | Dynamo České Budějovice | 2:1 | ||||
| 9. Round | 25/09/2021 | Dynamo České Budějovice | - | MFK Karviná | 3:1 | ||||
| 10. Round | 02/10/2021 | Dynamo České Budějovice | - | FK Pardubice | 3:1 | ||||
| 11. Round | 16/10/2021 | FK Jablonec | - | Dynamo České Budějovice | 2:2 | |
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| 13. Round | 30/10/2021 | FC Fastav Zlín | - | Dynamo České Budějovice | 2:0 | ||||
| 14. Round | 06/11/2021 | Dynamo České Budějovice | - | FK Mladá Boleslav | 2:1 | ||||
| 15. Round | 20/11/2021 | Bohemians Praha 1905 | - | Dynamo České Budějovice | 3:1 | |
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| 18. Round | 12/12/2021 | Dynamo České Budějovice | - | Sigma Olomouc | 2:1 | |
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| 19. Round | 19/12/2021 | Slovan Liberec | - | Dynamo České Budějovice | 0:0 | |
Ukraine