| 1. Round | 12/09/2020 | Port Vale FC | - | Crawley Town | 2:0 |  |
|||
| 2. Round | 19/09/2020 | Exeter City | - | Port Vale FC | 0:2 | ||||
| 3. Round | 26/09/2020 | Port Vale FC | - | Harrogate Town | 0:0 | ||||
| 4. Round | 03/10/2020 | Morecambe FC | - | Port Vale FC | 1:0 | ||||
| 11. Round | 17/11/2020 | Port Vale FC | - | Scunthorpe United | 0:1 | ||||
| 13. Round | 21/11/2020 | Newport County | - | Port Vale FC | 1:0 | ||||
| 14. Round | 24/11/2020 | Stevenage FC | - | Port Vale FC | 2:1 | ||||
| 15. Round | 28/11/2020 | Port Vale FC | - | Leyton Orient | 2:3 | ||||
| 25. Round | 23/01/2021 | Port Vale FC | - | Walsall FC | 1:3 | ||||
| 26. Round | 26/01/2021 | Scunthorpe United | - | Port Vale FC | 2:0 | ||||
| 31. Round | 23/02/2021 | Port Vale FC | - | Stevenage FC | 0:0 | ||||
| 32. Round | 27/02/2021 | Port Vale FC | - | Cambridge United | 0:1 | ||||
| 33. Round | 02/03/2021 | Salford City | - | Port Vale FC | 1:0 | ||||
| 36. Round | 13/03/2021 | Port Vale FC | - | Bolton Wanderers | 0:1 | ||||
| 39. Round | 02/04/2021 | Port Vale FC | - | Exeter City | 1:0 | ||||
| 40. Round | 05/04/2021 | Harrogate Town | - | Port Vale FC | 0:2 | ||||
| 42. Round | 17/04/2021 | Carlisle United | - | Port Vale FC | 0:0 | ||||
| 43. Round | 20/04/2021 | Barrow AFC | - | Port Vale FC | 0:2 |
England