| 1. Round | 12/09/2020 | Mansfield Town | - | Tranmere Rovers | 0:0 | ||||
| 2. Round | 19/09/2020 | Tranmere Rovers | - | Cheltenham Town | 0:3 | ||||
| 5. Round | 10/10/2020 | Salford City | - | Tranmere Rovers | 2:2 | ||||
| 7. Round | 20/10/2020 | Tranmere Rovers | - | Leyton Orient | 0:1 | ||||
| 8. Round | 24/10/2020 | Tranmere Rovers | - | Southend United | 2:0 | ||||
| 9. Round | 27/10/2020 | Crawley Town | - | Tranmere Rovers | 4:0 |
England