| 1. Round | 07/08/2021 | Stevenage FC | - | Barrow AFC | 1:0 | ||||
| 2. Round | 14/08/2021 | Barrow AFC | - | Hartlepool United | 3:2 | ||||
| 3. Round | 17/08/2021 | Barrow AFC | - | Exeter City | 0:0 |  |
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| 4. Round | 21/08/2021 | Harrogate Town | - | Barrow AFC | 2:1 | ||||
| 5. Round | 28/08/2021 | Barrow AFC | - | Bristol Rovers | 1:1 |  |
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| 9. Round | 24/09/2021 | Barrow AFC | - | Newport County | 2:1 | ||||
| 10. Round | 02/10/2021 | Mansfield Town | - | Barrow AFC | 0:1 | |
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| 11. Round | 09/10/2021 | Barrow AFC | - | Leyton Orient | 1:1 | ||||
| 12. Round | 16/10/2021 | Port Vale FC | - | Barrow AFC | 3:1 | ||||
| 13. Round | 19/10/2021 | Barrow AFC | - | Scunthorpe United | 1:1 | ||||
| 14. Round | 23/10/2021 | Walsall FC | - | Barrow AFC | 2:2 | ||||
| 15. Round | 30/10/2021 | Barrow AFC | - | Rochdale AFC | 1:2 | ||||
| 19. Round | 27/11/2021 | Sutton United | - | Barrow AFC | 1:0 | ||||
| 21. Round | 11/12/2021 | Barrow AFC | - | Swindon Town | 2:0 | ||||
| 23. Round | 26/12/2021 | Tranmere Rovers | - | Barrow AFC | 2:0 | ||||
| 25. Round | 01/01/2022 | Barrow AFC | - | Bradford City | 1:2 |
England