| 1. Round | 08/08/2009 | Sheffield Wednesday | - | Barnsley FC | 2:2 | ||||
| 3. Round | 18/08/2009 | Barnsley FC | - | Preston North End | 0:3 | ||||
| 4. Round | 22/08/2009 | Leicester City | - | Barnsley FC | 1:0 | ||||
| 6. Round | 12/09/2009 | Watford FC | - | Barnsley FC | 1:0 |  |
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| 8. Round | 19/09/2009 | Barnsley FC | - | Swansea City | 0:0 | ||||
| 41. Round | 03/04/2010 | Sheffield United | - | Barnsley FC | 0:0 | |
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| 42. Round | 05/04/2010 | Barnsley FC | - | Peterborough United | 2:2 | ||||
| 43. Round | 10/04/2010 | Barnsley FC | - | Derby County | 0:0 | ||||
| 44. Round | 17/04/2010 | Swansea City | - | Barnsley FC | 3:1 | |
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| 45. Round | 24/04/2010 | Barnsley FC | - | Queens Park Rangers | 0:1 | ||||
| 46. Round | 02/05/2010 | West Bromwich Albion | - | Barnsley FC | 1:1 |
England