| 1. Round | 09/08/2024 | Barnsley FC | - | Mansfield Town | 1:2 | ||||
| 2. Round | 17/08/2024 | Lincoln City | - | Barnsley FC | 1:2 | ||||
| 4. Round | 31/08/2024 | Crawley Town | - | Barnsley FC | 0:3 | ||||
| 5. Round | 07/09/2024 | Barnsley FC | - | Bristol Rovers | 2:1 | ||||
| 6. Round | 14/09/2024 | Stevenage FC | - | Barnsley FC | 3:0 | ||||
| 7. Round | 21/09/2024 | Burton Albion | - | Barnsley FC | 1:2 | ||||
| 8. Round | 28/09/2024 | Barnsley FC | - | Stockport County | 1:1 | ||||
| 9. Round | 01/10/2024 | Barnsley FC | - | Wycombe Wanderers | 2:2 | ||||
| 10. Round | 05/10/2024 | Huddersfield Town | - | Barnsley FC | 2:0 | ||||
| 12. Round | 19/10/2024 | Blackpool FC | - | Barnsley FC | 1:2 | ||||
| 13. Round | 22/10/2024 | Barnsley FC | - | Charlton Athletic | 2:2 | ||||
| 14. Round | 26/10/2024 | Shrewsbury Town | - | Barnsley FC | 0:2 | ||||
| 15. Round | 08/11/2024 | Barnsley FC | - | Rotherham United | 2:0 | ||||
| 21. Round | 21/12/2024 | Barnsley FC | - | Leyton Orient | 0:4 |
England