| 4. Round | 22/08/2009 | Leeds United | - | Tranmere Rovers | 3:0 | ||||
| 5. Round | 29/08/2009 | Tranmere Rovers | - | Charlton Athletic | 0:4 | ||||
| 7. Round | 12/09/2009 | Tranmere Rovers | - | Walsall FC | 2:3 | ||||
| 8. Round | 19/09/2009 | Exeter City | - | Tranmere Rovers | 2:1 |  |
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| 9. Round | 26/09/2009 | Tranmere Rovers | - | Colchester United | 1:1 | ||||
| 10. Round | 29/09/2009 | Wycombe Wanderers | - | Tranmere Rovers | 0:1 | ||||
| 11. Round | 03/10/2009 | Millwall FC | - | Tranmere Rovers | 5:0 | ||||
| 15. Round | 31/10/2009 | Tranmere Rovers | - | Swindon Town | 1:4 | ||||
| 16. Round | 14/11/2009 | Norwich City | - | Tranmere Rovers | 2:0 | ||||
| 17. Round | 21/11/2009 | Leyton Orient | - | Tranmere Rovers | 2:1 | ||||
| 19. Round | 01/12/2009 | Huddersfield Town | - | Tranmere Rovers | 3:3 | |
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| 21. Round | 12/12/2009 | Southampton FC | - | Tranmere Rovers | 3:0 |
England