| 1. Round | 12/09/2020 | Barrow AFC | - | Stevenage FC | 1:1 | ||||
| 2. Round | 19/09/2020 | Newport County | - | Barrow AFC | 2:1 |  |
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| 3. Round | 26/09/2020 | Barrow AFC | - | Colchester United | 1:1 |  |
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| 4. Round | 03/10/2020 | Carlisle United | - | Barrow AFC | 1:0 | ||||
| 5. Round | 10/10/2020 | Barrow AFC | - | Leyton Orient | 1:1 | ||||
| 7. Round | 20/10/2020 | Barrow AFC | - | Bolton Wanderers | 3:3 | |
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| 8. Round | 24/10/2020 | Barrow AFC | - | Walsall FC | 2:2 | |
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| 9. Round | 27/10/2020 | Mansfield Town | - | Barrow AFC | 2:4 | ||||
| 10. Round | 31/10/2020 | Barrow AFC | - | Bradford City | 1:0 | ||||
| 11. Round | 03/11/2020 | Grimsby Town | - | Barrow AFC | 1:0 | ||||
| 12. Round | 14/11/2020 | Cambridge United | - | Barrow AFC | 1:1 | ||||
| 13. Round | 21/11/2020 | Barrow AFC | - | Forest Green Rovers | 2:2 | ||||
| 14. Round | 24/11/2020 | Barrow AFC | - | Oldham Athletic | 3:4 | ||||
| 24. Round | 16/01/2021 | Barrow AFC | - | Scunthorpe United | 1:0 |
England