| 6. Round | 04/09/2021 | Tranmere Rovers | - | Hartlepool United | 1:0 | ||||
| 7. Round | 11/09/2021 | Rochdale AFC | - | Tranmere Rovers | 1:0 | ||||
| 9. Round | 25/09/2021 | Forest Green Rovers | - | Tranmere Rovers | 0:0 | ||||
| 10. Round | 02/10/2021 | Tranmere Rovers | - | Crawley Town | 2:1 | ||||
| 14. Round | 23/10/2021 | Tranmere Rovers | - | Northampton Town | 0:2 | ||||
| 17. Round | 20/11/2021 | Bristol Rovers | - | Tranmere Rovers | 2:2 | ||||
| 18. Round | 23/11/2021 | Tranmere Rovers | - | Bradford City | 2:1 | ||||
| 23. Round | 26/12/2021 | Tranmere Rovers | - | Barrow AFC | 2:0 |  |
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| 42. Round | 15/04/2022 | Bradford City | - | Tranmere Rovers | 1:1 |  |
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| 44. Round | 23/04/2022 | Stevenage FC | - | Tranmere Rovers | 2:0 |
England