| 7. Round | 20/10/2020 | Colchester United | - | Forest Green Rovers | 1:0 | ||||
| 8. Round | 24/10/2020 | Colchester United | - | Harrogate Town | 2:1 | ||||
| 15. Round | 01/12/2020 | Colchester United | - | Crawley Town | 1:1 | ||||
| 16. Round | 05/12/2020 | Colchester United | - | Grimsby Town | 2:1 | ||||
| 10. Round | 08/12/2020 | Scunthorpe United | - | Colchester United | 0:1 | ||||
| 17. Round | 12/12/2020 | Port Vale FC | - | Colchester United | 1:1 | ||||
| 20. Round | 26/12/2020 | Southend United | - | Colchester United | 2:0 | ||||
| 21. Round | 29/12/2020 | Colchester United | - | Cheltenham Town | 0:0 | ||||
| 24. Round | 16/01/2021 | Colchester United | - | Cambridge United | 1:1 | ||||
| 25. Round | 23/01/2021 | Morecambe FC | - | Colchester United | 3:0 | ||||
| 30. Round | 20/02/2021 | Crawley Town | - | Colchester United | 1:0 | ||||
| 33. Round | 02/03/2021 | Colchester United | - | Carlisle United | 2:1 | ||||
| 34. Round | 06/03/2021 | Colchester United | - | Newport County | 0:2 | ||||
| 35. Round | 09/03/2021 | Harrogate Town | - | Colchester United | 3:0 | ||||
| 41. Round | 09/04/2021 | Oldham Athletic | - | Colchester United | 5:2 |
England
Nigeria