| 1. Round | 07/08/2021 | Tranmere Rovers | - | Walsall FC | 1:0 | ||||
| 2. Round | 14/08/2021 | Port Vale FC | - | Tranmere Rovers | 0:0 | ||||
| 3. Round | 17/08/2021 | Swindon Town | - | Tranmere Rovers | 0:0 | ||||
| 4. Round | 21/08/2021 | Tranmere Rovers | - | Newport County | 0:1 | ||||
| 5. Round | 28/08/2021 | Scunthorpe United | - | Tranmere Rovers | 1:0 | ||||
| 15. Round | 30/10/2021 | Mansfield Town | - | Tranmere Rovers | 2:0 | ||||
| 16. Round | 13/11/2021 | Tranmere Rovers | - | Sutton United | 0:1 | ||||
| 17. Round | 20/11/2021 | Bristol Rovers | - | Tranmere Rovers | 2:2 |  |
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| 18. Round | 23/11/2021 | Tranmere Rovers | - | Bradford City | 2:1 | |
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| 26. Round | 08/01/2022 | Tranmere Rovers | - | Scunthorpe United | 4:0 | ||||
| 25. Round | 11/01/2022 | Salford City | - | Tranmere Rovers | 1:1 | |
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| 19. Round | 01/02/2022 | Tranmere Rovers | - | Stevenage FC | 1:0 | ||||
| 30. Round | 05/02/2022 | Barrow AFC | - | Tranmere Rovers | 1:1 | ||||
| 31. Round | 08/02/2022 | Tranmere Rovers | - | Swindon Town | 3:0 | |
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| 35. Round | 05/03/2022 | Northampton Town | - | Tranmere Rovers | 3:2 | ||||
| 36. Round | 11/03/2022 | Tranmere Rovers | - | Mansfield Town | 3:2 |
England