| 1. Round | 11/09/2020 | Arsenal FC U23 | - | Southampton FC U23 | 1:2 | ||||
| 2. Round | 18/09/2020 | Southampton FC U23 | - | Chelsea FC U23 | 1:4 | ||||
| 4. Round | 03/10/2020 | Tottenham Hotspur U23 | - | Southampton FC U23 | 2:1 | ||||
| 6. Round | 23/10/2020 | Derby County U23 | - | Southampton FC U23 | 3:1 | ||||
| 16. Round | 29/01/2021 | West Ham United U23 | - | Southampton FC U23 | 3:1 | ||||
| 23. Round | 09/04/2021 | Brighton & Hove Albion U23 | - | Southampton FC U23 | 3:0 | ||||
| 15. Round | 12/04/2021 | Chelsea FC U23 | - | Southampton FC U23 | 3:1 | ||||
| 24. Round | 19/04/2021 | Leicester City U23 | - | Southampton FC U23 | 7:1 | ||||
| 26. Round | 02/05/2021 | Southampton FC U23 | - | Everton FC U23 | 0:5 | ||||
| 13. Round | 07/05/2021 | Everton FC U23 | - | Southampton FC U23 | 4:0 |
England