| 7. Round | 12/09/2000 | Portsmouth FC | - | Tranmere Rovers | 2:0 |  |
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| 8. Round | 16/09/2000 | Tranmere Rovers | - | Sheffield Wednesday | 2:0 | ||||
| 12. Round | 14/10/2000 | Preston North End | - | Tranmere Rovers | 1:0 | ||||
| 13. Round | 17/10/2000 | Barnsley FC | - | Tranmere Rovers | 1:1 | ||||
| 14. Round | 21/10/2000 | Tranmere Rovers | - | West Bromwich Albion | 2:2 | ||||
| 15. Round | 25/10/2000 | Blackburn Rovers | - | Tranmere Rovers | 3:2 | ||||
| 17. Round | 04/11/2000 | Norwich City | - | Tranmere Rovers | 1:0 | ||||
| 21. Round | 02/12/2000 | Tranmere Rovers | - | Blackburn Rovers | 1:1 | ||||
| 22. Round | 09/12/2000 | Tranmere Rovers | - | Grimsby Town | 2:0 | ||||
| 23. Round | 16/12/2000 | Fulham FC | - | Tranmere Rovers | 3:1 |  |
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| 24. Round | 23/12/2000 | Tranmere Rovers | - | Wimbledon FC | 0:4 | ||||
| 25. Round | 26/12/2000 | Huddersfield Town | - | Tranmere Rovers | 3:0 | ||||
| 27. Round | 01/01/2001 | Tranmere Rovers | - | Sheffield United | 1:0 |
England