| 36. Round | 08/03/2025 | Fleetwood Town | - | Port Vale FC | 1:1 | ||||
| 29. Round | 11/03/2025 | Colchester United | - | Port Vale FC | 2:1 | ||||
| 37. Round | 15/03/2025 | Port Vale FC | - | Milton Keynes Dons | 3:0 | ||||
| 38. Round | 22/03/2025 | Port Vale FC | - | Morecambe FC | 1:0 | ||||
| 26. Round | 25/03/2025 | Port Vale FC | - | Barrow AFC | 0:1 | ||||
| 39. Round | 29/03/2025 | Crewe Alexandra | - | Port Vale FC | 0:1 | ||||
| 40. Round | 01/04/2025 | Port Vale FC | - | Bradford City | 2:0 | ||||
| 41. Round | 05/04/2025 | Walsall FC | - | Port Vale FC | 2:3 | ||||
| 42. Round | 12/04/2025 | Port Vale FC | - | Bromley FC | 5:0 | ||||
| 43. Round | 18/04/2025 | Carlisle United | - | Port Vale FC | 3:2 | ||||
| 44. Round | 21/04/2025 | Port Vale FC | - | Grimsby Town | 2:2 | ||||
| 45. Round | 26/04/2025 | AFC Wimbledon | - | Port Vale FC | 0:2 | ||||
| 46. Round | 03/05/2025 | Port Vale FC | - | Gillingham FC | 0:1 |
England