| 1. Round | 12/09/2020 | Cambridge United | - | Carlisle United | 3:0 | ||||
| 2. Round | 19/09/2020 | Morecambe FC | - | Cambridge United | 0:5 | ||||
| 3. Round | 26/09/2020 | Cambridge United | - | Tranmere Rovers | 0:0 |  |
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| 4. Round | 03/10/2020 | Exeter City | - | Cambridge United | 2:0 | ||||
| 9. Round | 27/10/2020 | Walsall FC | - | Cambridge United | 0:2 | ||||
| 10. Round | 31/10/2020 | Crawley Town | - | Cambridge United | 2:1 | ||||
| 11. Round | 03/11/2020 | Cambridge United | - | Salford City | 2:1 | ||||
| 12. Round | 14/11/2020 | Cambridge United | - | Barrow AFC | 1:1 | ||||
| 14. Round | 24/11/2020 | Cheltenham Town | - | Cambridge United | 1:1 | ||||
| 15. Round | 02/12/2020 | Cambridge United | - | Mansfield Town | 0:1 | ||||
| 16. Round | 05/12/2020 | Cambridge United | - | Oldham Athletic | 1:2 | ||||
| 17. Round | 12/12/2020 | Forest Green Rovers | - | Cambridge United | 2:0 | ||||
| 18. Round | 15/12/2020 | Cambridge United | - | Colchester United | 2:1 | ||||
| 19. Round | 19/12/2020 | Bradford City | - | Cambridge United | 1:0 | ||||
| 22. Round | 02/01/2021 | Grimsby Town | - | Cambridge United | 1:2 | ||||
| 24. Round | 16/01/2021 | Colchester United | - | Cambridge United | 1:1 | ||||
| 25. Round | 23/01/2021 | Cambridge United | - | Bradford City | 0:0 |
England