| 1. Round | 07/08/2021 | Carlisle United | - | Colchester United | 0:0 | ||||
| 2. Round | 14/08/2021 | Colchester United | - | Northampton Town | 0:1 | ||||
| 3. Round | 17/08/2021 | Colchester United | - | Mansfield Town | 1:1 | ||||
| 11. Round | 08/10/2021 | Tranmere Rovers | - | Colchester United | 2:0 |  |
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| 15. Round | 30/10/2021 | Colchester United | - | Scunthorpe United | 2:1 |
England