| 1. Round | 30/07/2022 | Northampton Town | - | Colchester United | 3:2 | ||||
| 2. Round | 06/08/2022 | Colchester United | - | Carlisle United | 1:1 | ||||
| 3. Round | 13/08/2022 | Stockport County | - | Colchester United | 1:0 | ||||
| 4. Round | 16/08/2022 | Colchester United | - | Bradford City | 1:0 | ||||
| 6. Round | 27/08/2022 | Tranmere Rovers | - | Colchester United | 2:0 | ||||
| 9. Round | 13/09/2022 | Walsall FC | - | Colchester United | 1:1 | ||||
| 10. Round | 17/09/2022 | Colchester United | - | Grimsby Town | 0:1 | ||||
| 11. Round | 24/09/2022 | Colchester United | - | Rochdale AFC | 0:1 | ||||
| 15. Round | 15/10/2022 | Swindon Town | - | Colchester United | 1:0 | ||||
| 13. Round | 25/10/2022 | Colchester United | - | Crawley Town | 2:2 | ||||
| 17. Round | 29/10/2022 | Colchester United | - | Stevenage FC | 1:1 | ||||
| 8. Round | 01/11/2022 | Barrow AFC | - | Colchester United | 3:1 |
England