| 1. Round | 07/08/2021 | Tranmere Rovers | - | Walsall FC | 1:0 | ||||
| 2. Round | 14/08/2021 | Port Vale FC | - | Tranmere Rovers | 0:0 | ||||
| 3. Round | 17/08/2021 | Swindon Town | - | Tranmere Rovers | 0:0 | ||||
| 4. Round | 21/08/2021 | Tranmere Rovers | - | Newport County | 0:1 | ||||
| 5. Round | 28/08/2021 | Scunthorpe United | - | Tranmere Rovers | 1:0 | ||||
| 6. Round | 04/09/2021 | Tranmere Rovers | - | Hartlepool United | 1:0 | ||||
| 7. Round | 11/09/2021 | Rochdale AFC | - | Tranmere Rovers | 1:0 |  |
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| 9. Round | 25/09/2021 | Forest Green Rovers | - | Tranmere Rovers | 0:0 | ||||
| 11. Round | 08/10/2021 | Tranmere Rovers | - | Colchester United | 2:0 | ||||
| 12. Round | 16/10/2021 | Carlisle United | - | Tranmere Rovers | 0:1 | ||||
| 14. Round | 23/10/2021 | Tranmere Rovers | - | Northampton Town | 0:2 | ||||
| 16. Round | 13/11/2021 | Tranmere Rovers | - | Sutton United | 0:1 | ||||
| 18. Round | 23/11/2021 | Tranmere Rovers | - | Bradford City | 2:1 | ||||
| 22. Round | 18/12/2021 | Tranmere Rovers | - | Leyton Orient | 1:0 | ||||
| 23. Round | 26/12/2021 | Tranmere Rovers | - | Barrow AFC | 2:0 | ||||
| 26. Round | 08/01/2022 | Tranmere Rovers | - | Scunthorpe United | 4:0 |  |
England