| 1. Round | 12/09/2020 | Barrow AFC | - | Stevenage FC | 1:1 | ||||
| 2. Round | 19/09/2020 | Newport County | - | Barrow AFC | 2:1 | ||||
| 8. Round | 24/10/2020 | Barrow AFC | - | Walsall FC | 2:2 | ||||
| 9. Round | 27/10/2020 | Mansfield Town | - | Barrow AFC | 2:4 | ||||
| 10. Round | 31/10/2020 | Barrow AFC | - | Bradford City | 1:0 | ||||
| 11. Round | 03/11/2020 | Grimsby Town | - | Barrow AFC | 1:0 |  |
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| 12. Round | 14/11/2020 | Cambridge United | - | Barrow AFC | 1:1 | ||||
| 13. Round | 21/11/2020 | Barrow AFC | - | Forest Green Rovers | 2:2 | ||||
| 15. Round | 01/12/2020 | Morecambe FC | - | Barrow AFC | 1:0 |  |
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| 17. Round | 12/12/2020 | Crawley Town | - | Barrow AFC | 4:2 |  |
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| 18. Round | 15/12/2020 | Scunthorpe United | - | Barrow AFC | 2:1 | ||||
| 19. Round | 19/12/2020 | Barrow AFC | - | Cheltenham Town | 3:0 | ||||
| 20. Round | 26/12/2020 | Port Vale FC | - | Barrow AFC | 0:2 | ||||
| 21. Round | 29/12/2020 | Barrow AFC | - | Tranmere Rovers | 1:1 | ||||
| 23. Round | 09/01/2021 | Southend United | - | Barrow AFC | 1:0 | |
England