| 2. Round | 22/08/2016 | Southampton FC U23 | - | Manchester United U23 | 2:0 | ||||
| 4. Round | 12/09/2016 | Southampton FC U23 | - | Reading FC U23 | 3:1 | ||||
| 5. Round | 19/09/2016 | Southampton FC U23 | - | Leicester City U23 | 2:2 | ||||
| 6. Round | 23/09/2016 | Southampton FC U23 | - | Tottenham Hotspur U23 | 1:0 | ||||
| 17. Round | 06/02/2017 | Leicester City U23 | - | Southampton FC U23 | 0:2 | ||||
| 19. Round | 06/03/2017 | Southampton FC U23 | - | Chelsea FC U23 | 0:0 | ||||
| 20. Round | 13/03/2017 | Manchester United U23 | - | Southampton FC U23 | 3:3 | ||||
| 21. Round | 10/04/2017 | Southampton FC U23 | - | Liverpool FC U23 | 2:2 | ||||
| 22. Round | 21/04/2017 | Southampton FC U23 | - | Derby County U23 | 0:1 | ||||
| 13. Round | 05/05/2017 | Manchester City U23 | - | Southampton FC U23 | 4:2 |  |
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England