| 1. Round | 08/08/2009 | Port Vale FC | - | Rochdale AFC | 1:1 | ||||
| 2. Round | 15/08/2009 | Bradford City | - | Port Vale FC | 0:0 | ||||
| 3. Round | 18/08/2009 | Macclesfield Town | - | Port Vale FC | 2:0 | ||||
| 9. Round | 26/09/2009 | Notts County | - | Port Vale FC | 3:1 | ||||
| 10. Round | 29/09/2009 | Port Vale FC | - | Accrington Stanley | 2:2 | ||||
| 12. Round | 10/10/2009 | Shrewsbury Town | - | Port Vale FC | 0:1 |  |
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| 13. Round | 17/10/2009 | Crewe Alexandra | - | Port Vale FC | 1:2 | ||||
| 14. Round | 24/10/2009 | Port Vale FC | - | Cheltenham Town | 1:1 | |
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| 15. Round | 31/10/2009 | Dagenham & Redbridge | - | Port Vale FC | 1:1 | |
England