| 11. Round | 08/10/2021 | Tranmere Rovers | - | Colchester United | 2:0 | ||||
| 12. Round | 16/10/2021 | Colchester United | - | Harrogate Town | 1:0 | ||||
| 13. Round | 19/10/2021 | Colchester United | - | Bristol Rovers | 1:1 | ||||
| 14. Round | 23/10/2021 | Port Vale FC | - | Colchester United | 3:0 | ||||
| 6. Round | 26/10/2021 | Colchester United | - | Sutton United | 1:3 |  |
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| 16. Round | 11/01/2022 | Forest Green Rovers | - | Colchester United | 2:0 |
England