| 26. Round | 01/02/2022 | Colchester United | - | Rochdale AFC | 1:1 | ||||
| 30. Round | 05/02/2022 | Leyton Orient | - | Colchester United | 0:1 | ||||
| 31. Round | 08/02/2022 | Mansfield Town | - | Colchester United | 2:1 | ||||
| 32. Round | 12/02/2022 | Colchester United | - | Carlisle United | 2:2 | ||||
| 33. Round | 19/02/2022 | Northampton Town | - | Colchester United | 3:0 | ||||
| 22. Round | 22/02/2022 | Colchester United | - | Hartlepool United | 1:2 | ||||
| 45. Round | 30/04/2022 | Colchester United | - | Walsall FC | 2:2 | ||||
| 46. Round | 07/05/2022 | Hartlepool United | - | Colchester United | 0:2 |
England