| 1. Round | 30/07/2022 | Northampton Town | - | Colchester United | 3:2 | ||||
| 2. Round | 06/08/2022 | Colchester United | - | Carlisle United | 1:1 | ||||
| 3. Round | 13/08/2022 | Stockport County | - | Colchester United | 1:0 | ||||
| 4. Round | 16/08/2022 | Colchester United | - | Bradford City | 1:0 | ||||
| 5. Round | 20/08/2022 | Colchester United | - | Leyton Orient | 1:3 | ||||
| 14. Round | 08/10/2022 | Colchester United | - | Harrogate Town | 2:1 | ||||
| 15. Round | 15/10/2022 | Swindon Town | - | Colchester United | 1:0 | ||||
| 16. Round | 22/10/2022 | Newport County | - | Colchester United | 1:0 | ||||
| 13. Round | 25/10/2022 | Colchester United | - | Crawley Town | 2:2 | ||||
| 17. Round | 29/10/2022 | Colchester United | - | Stevenage FC | 1:1 | ||||
| 8. Round | 01/11/2022 | Barrow AFC | - | Colchester United | 3:1 | ||||
| 18. Round | 12/11/2022 | Crewe Alexandra | - | Colchester United | 1:0 | ||||
| 19. Round | 19/11/2022 | Colchester United | - | Doncaster Rovers | 3:0 |
England