1. Round 12/09/2020 Mansfield Town - Tranmere Rovers 0:0
2. Round 19/09/2020 Tranmere Rovers - Cheltenham Town 0:3
3. Round 26/09/2020 Cambridge United - Tranmere Rovers 0:0
4. Round 03/10/2020 Tranmere Rovers - Scunthorpe United 2:0 yellow card
8. Round 24/10/2020 Tranmere Rovers - Southend United 2:0
9. Round 27/10/2020 Crawley Town - Tranmere Rovers 4:0
10. Round 31/10/2020 Tranmere Rovers - Morecambe FC 0:1
11. Round 03/11/2020 Harrogate Town - Tranmere Rovers 0:1
12. Round 14/11/2020 Port Vale FC - Tranmere Rovers 3:4 yellow card
13. Round 21/11/2020 Tranmere Rovers - Grimsby Town 5:0
14. Round 24/11/2020 Tranmere Rovers - Carlisle United 1:0
15. Round 01/12/2020 Oldham Athletic - Tranmere Rovers 0:1
16. Round 05/12/2020 Tranmere Rovers - Walsall FC 1:3
17. Round 12/12/2020 Exeter City - Tranmere Rovers 5:0 yellow card
19. Round 19/12/2020 Bolton Wanderers - Tranmere Rovers 0:3
20. Round 26/12/2020 Tranmere Rovers - Bradford City 0:1
21. Round 29/12/2020 Barrow AFC - Tranmere Rovers 1:1
24. Round 16/01/2021 Stevenage FC - Tranmere Rovers 0:0
23. Round 19/01/2021 Tranmere Rovers - Forest Green Rovers 3:2 yellow card
25. Round 23/01/2021 Tranmere Rovers - Bolton Wanderers 2:1
26. Round 26/01/2021 Tranmere Rovers - Harrogate Town 3:2
27. Round 30/01/2021 Morecambe FC - Tranmere Rovers 0:1
28. Round 06/02/2021 Tranmere Rovers - Port Vale FC 3:1
18. Round 09/02/2021 Tranmere Rovers - Stevenage FC 0:1
30. Round 20/02/2021 Tranmere Rovers - Oldham Athletic 2:2
31. Round 23/02/2021 Carlisle United - Tranmere Rovers 2:3
32. Round 27/02/2021 Leyton Orient - Tranmere Rovers 1:3
33. Round 02/03/2021 Tranmere Rovers - Newport County 1:0
34. Round 06/03/2021 Tranmere Rovers - Crawley Town 0:1
35. Round 09/03/2021 Southend United - Tranmere Rovers 0:2
29. Round 17/03/2021 Grimsby Town - Tranmere Rovers 0:0
37. Round 20/03/2021 Tranmere Rovers - Exeter City 2:1
22. Round 23/03/2021 Colchester United - Tranmere Rovers 2:2
38. Round 27/03/2021 Tranmere Rovers - Mansfield Town 1:1