| 25. Round | 26/01/2010 | Millwall FC | - | Southend United | 2:0 |  |
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| 29. Round | 30/01/2010 | Brighton & Hove Albion | - | Millwall FC | 0:1 | ||||
| 31. Round | 13/02/2010 | Millwall FC | - | Exeter City | 1:0 | ||||
| 32. Round | 20/02/2010 | Wycombe Wanderers | - | Millwall FC | 1:0 | ||||
| 34. Round | 27/02/2010 | Millwall FC | - | Hartlepool United | 1:0 | ||||
| 35. Round | 06/03/2010 | Walsall FC | - | Millwall FC | 2:2 | ||||
| 26. Round | 09/03/2010 | Carlisle United | - | Millwall FC | 1:3 | ||||
| 36. Round | 13/03/2010 | Millwall FC | - | Charlton Athletic | 4:0 | ||||
| 37. Round | 22/03/2010 | Leeds United | - | Millwall FC | 0:2 | |
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| 38. Round | 27/03/2010 | Millwall FC | - | Stockport County | 5:0 | ||||
| 39. Round | 02/04/2010 | Millwall FC | - | Brentford FC | 1:1 | ||||
| 40. Round | 05/04/2010 | Colchester United | - | Millwall FC | 1:2 | ||||
| 41. Round | 10/04/2010 | Millwall FC | - | Gillingham FC | 4:0 | |
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| 42. Round | 13/04/2010 | Yeovil Town | - | Millwall FC | 1:1 | ||||
| 43. Round | 16/04/2010 | Huddersfield Town | - | Millwall FC | 1:0 | ||||
| 46. Round | 08/05/2010 | Millwall FC | - | Swindon Town | 3:2 |
England