| 27. Round | 30/01/2021 | Cambridge United | - | Crawley Town | 3:1 |  |
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| 28. Round | 06/02/2021 | Barrow AFC | - | Cambridge United | 0:2 | ||||
| 26. Round | 09/02/2021 | Salford City | - | Cambridge United | 4:1 | ||||
| 29. Round | 13/02/2021 | Cambridge United | - | Southend United | 0:0 | ||||
| 31. Round | 23/02/2021 | Cambridge United | - | Cheltenham Town | 0:1 | ||||
| 32. Round | 27/02/2021 | Port Vale FC | - | Cambridge United | 0:1 | ||||
| 33. Round | 02/03/2021 | Cambridge United | - | Scunthorpe United | 0:1 | ||||
| 34. Round | 06/03/2021 | Cambridge United | - | Walsall FC | 1:0 | ||||
| 35. Round | 09/03/2021 | Bolton Wanderers | - | Cambridge United | 2:1 | ||||
| 37. Round | 20/03/2021 | Cambridge United | - | Forest Green Rovers | 1:0 | ||||
| 38. Round | 27/03/2021 | Carlisle United | - | Cambridge United | 1:2 | ||||
| 39. Round | 02/04/2021 | Cambridge United | - | Morecambe FC | 2:1 | ||||
| 41. Round | 10/04/2021 | Cambridge United | - | Exeter City | 1:4 | ||||
| 42. Round | 17/04/2021 | Newport County | - | Cambridge United | 0:1 | ||||
| 43. Round | 20/04/2021 | Leyton Orient | - | Cambridge United | 2:4 |  |
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| 44. Round | 24/04/2021 | Cambridge United | - | Stevenage FC | 0:1 | ||||
| 45. Round | 30/04/2021 | Harrogate Town | - | Cambridge United | 5:4 |
England