| 1. Round | 12/08/2000 | Grimsby Town | - | Preston North End | 1:2 | ||||
| 2. Round | 19/08/2000 | Preston North End | - | Sheffield United | 3:0 | ||||
| 3. Round | 26/08/2000 | Bolton Wanderers | - | Preston North End | 2:0 | ||||
| 4. Round | 28/08/2000 | Preston North End | - | Wimbledon FC | 1:1 | ||||
| 5. Round | 02/09/2000 | Preston North End | - | Portsmouth FC | 1:0 |  |
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| 6. Round | 09/09/2000 | Queens Park Rangers | - | Preston North End | 0:0 | ||||
| 7. Round | 12/09/2000 | Birmingham City | - | Preston North End | 3:1 | ||||
| 8. Round | 16/09/2000 | Preston North End | - | Stockport County | 1:1 | ||||
| 9. Round | 23/09/2000 | Sheffield Wednesday | - | Preston North End | 1:3 | ||||
| 10. Round | 30/09/2000 | Preston North End | - | Crystal Palace | 2:0 | ||||
| 12. Round | 14/10/2000 | Preston North End | - | Tranmere Rovers | 1:0 | |
England