| 2. Round | 16/03/2024 | El Paso Locomotive | - | Louisville City FC | 0:1 | ||||
| 5. Round | 06/04/2024 | Louisville City FC | - | Indy Eleven | 5:3 | ||||
| 6. Round | 10/04/2024 | Charleston Battery | - | Louisville City FC | 3:2 | ||||
| 8. Round | 28/04/2024 | Louisville City FC | - | Hartford Athletic | 6:0 |  |
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| 11. Round | 19/05/2024 | Las Vegas Lights FC | - | Louisville City FC | 2:2 | ||||
| 13. Round | 02/06/2024 | Miami FC | - | Louisville City FC | 1:2 | ||||
| 16. Round | 20/06/2024 | Pittsburgh Riverhounds | - | Louisville City FC | 0:1 | ||||
| 16. Round | 23/06/2024 | Louisville City FC | - | Rhode Island FC | 2:5 | ||||
| 17. Round | 30/06/2024 | Birmingham Legion | - | Louisville City FC | 1:4 | |
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| 18. Round | 07/07/2024 | Oakland Roots | - | Louisville City FC | 1:0 |
England