| 1. Round | 07/08/2021 | Northampton Town | - | Port Vale FC | 1:0 | ||||
| 5. Round | 28/08/2021 | Forest Green Rovers | - | Port Vale FC | 0:2 | ||||
| 6. Round | 04/09/2021 | Port Vale FC | - | Rochdale AFC | 2:3 | ||||
| 7. Round | 11/09/2021 | Swindon Town | - | Port Vale FC | 1:2 |  |
|||
| 8. Round | 18/09/2021 | Port Vale FC | - | Harrogate Town | 2:0 | |
|||
| 9. Round | 25/09/2021 | Scunthorpe United | - | Port Vale FC | 0:1 | ||||
| 10. Round | 02/10/2021 | Port Vale FC | - | Leyton Orient | 3:2 | ||||
| 11. Round | 09/10/2021 | Sutton United | - | Port Vale FC | 4:3 |  |
|||
| 12. Round | 16/10/2021 | Port Vale FC | - | Barrow AFC | 3:1 | ||||
| 13. Round | 19/10/2021 | Mansfield Town | - | Port Vale FC | 1:1 | |
|||
| 14. Round | 23/10/2021 | Port Vale FC | - | Colchester United | 3:0 | ||||
| 15. Round | 30/10/2021 | Crawley Town | - | Port Vale FC | 1:4 | |
|||
| 16. Round | 13/11/2021 | Port Vale FC | - | Bradford City | 1:1 | ||||
| 17. Round | 20/11/2021 | Oldham Athletic | - | Port Vale FC | 3:2 | ||||
| 18. Round | 23/11/2021 | Port Vale FC | - | Walsall FC | 0:1 |  |
|||
| 20. Round | 07/12/2021 | Bristol Rovers | - | Port Vale FC | 1:2 | ||||
| 21. Round | 11/12/2021 | Newport County | - | Port Vale FC | 2:1 | ||||
| 27. Round | 15/01/2022 | Port Vale FC | - | Swindon Town | 1:3 |
England