| 27. Round | 15/01/2022 | Colchester United | - | Barrow AFC | 0:2 |  |
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| 24. Round | 18/01/2022 | Sutton United | - | Colchester United | 3:2 | |
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| 28. Round | 22/01/2022 | Salford City | - | Colchester United | 0:3 | ||||
| 29. Round | 29/01/2022 | Colchester United | - | Swindon Town | 1:1 | ||||
| 26. Round | 01/02/2022 | Colchester United | - | Rochdale AFC | 1:1 | ||||
| 34. Round | 26/02/2022 | Colchester United | - | Oldham Athletic | 1:1 | ||||
| 23. Round | 01/03/2022 | Colchester United | - | Leyton Orient | 2:2 | ||||
| 35. Round | 05/03/2022 | Colchester United | - | Port Vale FC | 1:0 |  |
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| 39. Round | 26/03/2022 | Colchester United | - | Tranmere Rovers | 1:0 |
England