| 1. Round | 10/08/2024 | AFC Wimbledon | - | Colchester United | 4:2 | ||||
| 2. Round | 17/08/2024 | Colchester United | - | Milton Keynes Dons | 2:0 | ||||
| 3. Round | 24/08/2024 | Colchester United | - | Harrogate Town | 0:1 | ||||
| 4. Round | 31/08/2024 | Accrington Stanley | - | Colchester United | 1:1 | ||||
| 5. Round | 07/09/2024 | Colchester United | - | Bromley FC | 1:1 | ||||
| 6. Round | 14/09/2024 | Morecambe FC | - | Colchester United | 3:3 | ||||
| 9. Round | 01/10/2024 | Port Vale FC | - | Colchester United | 1:1 | ||||
| 10. Round | 05/10/2024 | Colchester United | - | Carlisle United | 0:0 | ||||
| 12. Round | 19/10/2024 | Colchester United | - | Cheltenham Town | 1:2 | ||||
| 13. Round | 22/10/2024 | Chesterfield FC | - | Colchester United | 1:1 | ||||
| 14. Round | 26/10/2024 | Colchester United | - | Salford City | 1:2 | ||||
| 16. Round | 16/11/2024 | Colchester United | - | Bradford City | 1:1 | ||||
| 21. Round | 20/12/2024 | Crewe Alexandra | - | Colchester United | 0:0 | ||||
| 22. Round | 26/12/2024 | Colchester United | - | Gillingham FC | 2:0 | ||||
| 24. Round | 01/01/2025 | Swindon Town | - | Colchester United | 3:2 | ||||
| 27. Round | 18/01/2025 | Bromley FC | - | Colchester United | 0:1 |
England
