| 1. Round | 12/09/2020 | Scunthorpe United | - | Newport County | 1:1 | ||||
| 2. Round | 19/09/2020 | Crawley Town | - | Scunthorpe United | 1:0 | ||||
| 3. Round | 26/09/2020 | Scunthorpe United | - | Carlisle United | 1:0 | ||||
| 4. Round | 03/10/2020 | Tranmere Rovers | - | Scunthorpe United | 2:0 | ||||
| 5. Round | 10/10/2020 | Scunthorpe United | - | Forest Green Rovers | 1:4 | ||||
| 6. Round | 17/10/2020 | Scunthorpe United | - | Cambridge United | 0:5 | ||||
| 7. Round | 20/10/2020 | Cheltenham Town | - | Scunthorpe United | 1:0 | ||||
| 18. Round | 15/12/2020 | Scunthorpe United | - | Barrow AFC | 2:1 | ||||
| 20. Round | 26/12/2020 | Scunthorpe United | - | Mansfield Town | 2:3 | ||||
| 21. Round | 29/12/2020 | Walsall FC | - | Scunthorpe United | 1:2 | ||||
| 22. Round | 02/01/2021 | Stevenage FC | - | Scunthorpe United | 3:1 | ||||
| 9. Round | 12/01/2021 | Scunthorpe United | - | Salford City | 0:1 |
England