| 22. Round | 25/01/2022 | Scunthorpe United | - | Bristol Rovers | 2:3 | ||||
| 29. Round | 29/01/2022 | Port Vale FC | - | Scunthorpe United | 1:0 | ||||
| 30. Round | 05/02/2022 | Scunthorpe United | - | Oldham Athletic | 0:1 | ||||
| 31. Round | 08/02/2022 | Scunthorpe United | - | Walsall FC | 1:0 | ||||
| 32. Round | 12/02/2022 | Swindon Town | - | Scunthorpe United | 3:0 | ||||
| 33. Round | 19/02/2022 | Scunthorpe United | - | Rochdale AFC | 1:2 | ||||
| 24. Round | 22/02/2022 | Scunthorpe United | - | Northampton Town | 0:0 | ||||
| 34. Round | 26/02/2022 | Sutton United | - | Scunthorpe United | 4:1 | ||||
| 36. Round | 12/03/2022 | Scunthorpe United | - | Colchester United | 1:3 | ||||
| 37. Round | 15/03/2022 | Scunthorpe United | - | Barrow AFC | 0:1 | ||||
| 38. Round | 19/03/2022 | Salford City | - | Scunthorpe United | 5:1 | ||||
| 39. Round | 26/03/2022 | Scunthorpe United | - | Harrogate Town | 0:3 | ||||
| 40. Round | 02/04/2022 | Forest Green Rovers | - | Scunthorpe United | 1:0 | ||||
| 42. Round | 15/04/2022 | Leyton Orient | - | Scunthorpe United | 3:0 |
England