14/11/1964 Chester City - Crewe Alexandra 5:0
Chesterfield FC - South Shields 2:0
Dartford FC - Aldershot FC 1:1
Bournemouth and Boscombe Athletic - Gravesend & Northfleet 7:0
Barnet FC - Cambridge United 2:1
Barrow AFC - Grimsby Town 1:1
Bristol City - Brighton & Hove Albion 1:0
Welton Rovers - Weymouth FC 1:1
Reading FC - Watford FC 3:1
Walsall FC - Bristol Rovers 0:2
Wisbech Town - Brentford FC 0:2
Notts County - Chelmsford City 2:0
Macclesfield Town - Wrexham AFC 1:2
Luton Town - Southend United 1:0
Scarborough FC - Bradford City 1:0
Tranmere Rovers - Lincoln City 0:0
Stockport County - Wigan Athletic 2:1
Kidderminster Harriers - Hull City 1:4
Queens Park Rangers - Bath City FC 2:0
Crook Town AFC - Carlisle United 1:0
King's Lynn - Shrewsbury Town 0:1
Millwall FC - Kettering Town 2:0
Oldham Athletic - Hereford United 4:0
Bradford Park Avenue - Doncaster Rovers 2:3
Exeter City - Hayes FC 1:0
Scunthorpe United - Darlington FC 1:2
Port Vale FC - Hendon FC 2:1
Halifax Town - South Liverpool 2:2
Newport County - Spalding United 5:3
Southport FC - Annfield Plain 6:1
Workington AFC - Rochdale AFC 2:0
York City - Bangor City 5:1
Netherfield - Barnsley FC 1:3
Guildford City - Gillingham FC 2:2
Romford FC - Enfield FC 0:0
Peterborough United - Salisbury City 5:1
Colchester United - Bideford AFC 3:3
Canterbury City - Torquay United 0:6
Corby Town - Hartlepools United 1:3
Oxford United - Mansfield Town 0:1
decisive matches
17/11/1964 Grimsby Town - Barrow AFC 2:2
Enfield FC - Romford FC 0:0
18/11/1964 Aldershot FC - Dartford FC 1:0
Weymouth FC - Welton Rovers 4:3
Lincoln City - Tranmere Rovers 1:0
South Liverpool - Halifax Town 4:2
Gillingham FC - Guildford City 1:0
Bideford AFC - Colchester United 1:2
23/11/1964 Barrow AFC - Grimsby Town 0:2
Romford FC - Enfield FC 2:4