| 1. Round | 06/09/1965 | Sampdoria | - | Torino FC | 1:1 | ||||
| 2. Round | 12/09/1965 | Cagliari Calcio | - | Sampdoria | 1:1 | ||||
| 3. Round | 19/09/1965 | Sampdoria | - | AS Roma | 0:1 | ||||
| 4. Round | 26/09/1965 | AC Milan | - | Sampdoria | 2:0 | ||||
| 5. Round | 03/10/1965 | Sampdoria | - | US Foggia | 2:1 | ||||
| 6. Round | 10/10/1965 | Brescia Calcio | - | Sampdoria | 1:0 | ||||
| 7. Round | 17/10/1965 | Sampdoria | - | Bologna FC | 0:2 | ||||
| 8. Round | 24/10/1965 | ACF Fiorentina | - | Sampdoria | 5:0 | ||||
| 9. Round | 14/11/1965 | Sampdoria | - | Calcio Catania | 1:0 | ||||
| 10. Round | 21/11/1965 | AC Lanerossi Vicenza | - | Sampdoria | 2:1 | ||||
| 11. Round | 28/11/1965 | Sampdoria | - | AS Varese 1910 | 2:0 | ||||
| 24. Round | 06/03/1966 | Bologna FC | - | Sampdoria | 2:1 | ||||
| 25. Round | 13/03/1966 | Sampdoria | - | ACF Fiorentina | 0:3 |
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