| 1. Round | 27/07/2019 | TSV Steinbach Haiger | - | 1. FSV Mainz 05 II | 3:0 |  |
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| 2. Round | 04/08/2019 | 1. FSV Mainz 05 II | - | Rot-Weiß Koblenz | 2:0 | ||||
| 3. Round | 10/08/2019 | FSV Frankfurt | - | 1. FSV Mainz 05 II | 1:1 |  |
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| 7. Round | 31/08/2019 | 1. FSV Mainz 05 II | - | 1. FC Saarbrücken | 0:2 | ||||
| 8. Round | 07/09/2019 | Bayern Alzenau | - | 1. FSV Mainz 05 II | 0:3 | ||||
| 9. Round | 15/09/2019 | 1. FSV Mainz 05 II | - | FC Gießen | 1:3 | ||||
| 10. Round | 22/09/2019 | SSV Ulm 1846 | - | 1. FSV Mainz 05 II | 0:1 | ||||
| 11. Round | 29/09/2019 | 1. FSV Mainz 05 II | - | SC Freiburg II | 3:2 | ||||
| 12. Round | 05/10/2019 | VfR Aalen | - | 1. FSV Mainz 05 II | 0:0 | ||||
| 14. Round | 19/10/2019 | Kickers Offenbach | - | 1. FSV Mainz 05 II | 1:3 | |
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| 15. Round | 26/10/2019 | 1. FSV Mainz 05 II | - | SV 07 Elversberg | 1:2 | |
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| 16. Round | 02/11/2019 | FC 08 Homburg | - | 1. FSV Mainz 05 II | 2:1 | ||||
| 17. Round | 08/11/2019 | 1. FSV Mainz 05 II | - | FK Pirmasens | 4:4 | |
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| 18. Round | 23/11/2019 | 1. FSV Mainz 05 II | - | TSV Steinbach Haiger | 0:2 | |
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| 20. Round | 07/12/2019 | 1. FSV Mainz 05 II | - | FSV Frankfurt | 2:1 |
Germany