| 1. Round | 21/08/2009 | AC Mantova | - | US Triestina | 0:0 |  |
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| 2. Round | 29/08/2009 | US Triestina | - | US Grosseto FC | 1:0 | ||||
| 4. Round | 12/09/2009 | US Triestina | - | US Salernitana 1919 | 2:0 | ||||
| 5. Round | 19/09/2009 | AC Cesena | - | US Triestina | 4:1 | |
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| 6. Round | 22/09/2009 | US Triestina | - | US Lecce | 1:4 |  |
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| 8. Round | 03/10/2009 | US Triestina | - | UC AlbinoLeffe | 2:3 | ||||
| 16. Round | 28/11/2009 | Modena FC | - | US Triestina | 2:0 | ||||
| 25. Round | 15/02/2010 | US Salernitana 1919 | - | US Triestina | 1:2 | ||||
| 26. Round | 20/02/2010 | US Triestina | - | AC Cesena | 0:0 | ||||
| 27. Round | 27/02/2010 | US Lecce | - | US Triestina | 1:1 | ||||
| 28. Round | 06/03/2010 | US Triestina | - | Vicenza Calcio | 0:0 | |
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| 30. Round | 20/03/2010 | US Triestina | - | Empoli FC | 1:0 | ||||
| 31. Round | 23/03/2010 | Gallipoli | - | US Triestina | 2:1 | |
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| 37. Round | 26/04/2010 | US Triestina | - | Modena FC | 1:1 | ||||
| 38. Round | 01/05/2010 | FC Crotone | - | US Triestina | 2:0 |
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