| 22. Round | 03/02/2021 | US Alessandria 1912 | - | Calcio Lecco 1912 | 0:0 |  |
|||
| 23. Round | 07/02/2021 | Novara Calcio | - | US Alessandria 1912 | 2:1 | ||||
| 24. Round | 13/02/2021 | US Alessandria 1912 | - | Giana Erminio | 0:0 | ||||
| 25. Round | 17/02/2021 | US Grosseto FC | - | US Alessandria 1912 | 1:1 | ||||
| 26. Round | 21/02/2021 | US Pontedera | - | US Alessandria 1912 | 0:0 | ||||
| 27. Round | 01/03/2021 | US Alessandria 1912 | - | Carrarese Calcio | 1:0 | ||||
| 28. Round | 04/03/2021 | AS Livorno | - | US Alessandria 1912 | 0:1 | ||||
| 29. Round | 07/03/2021 | US Alessandria 1912 | - | UC AlbinoLeffe | 2:1 | ||||
| 30. Round | 14/03/2021 | Piacenza Calcio | - | US Alessandria 1912 | 1:0 | |
|||
| 31. Round | 18/03/2021 | US Alessandria 1912 | - | AC Renate | 2:0 | ||||
| 32. Round | 22/03/2021 | Pro Vercelli | - | US Alessandria 1912 | 0:1 |
Italy