| 7. Round | 06/09/2021 | VfL Osnabrück | - | Viktoria Köln | 3:0 | ||||
| 8. Round | 12/09/2021 | Eintracht Braunschweig | - | VfL Osnabrück | 2:2 | ||||
| 10. Round | 25/09/2021 | 1. FC Kaiserslautern | - | VfL Osnabrück | 2:0 |  |
|||
| 11. Round | 02/10/2021 | VfL Osnabrück | - | FSV Zwickau | 0:1 | ||||
| 13. Round | 22/10/2021 | VfL Osnabrück | - | Hallescher FC | 0:0 | ||||
| 15. Round | 06/11/2021 | VfL Osnabrück | - | TSV 1860 München | 3:1 | ||||
| 16. Round | 21/11/2021 | Waldhof Mannheim | - | VfL Osnabrück | 3:3 | ||||
| 17. Round | 28/11/2021 | VfL Osnabrück | - | SC Freiburg II | 0:1 | ||||
| 21. Round | 15/01/2022 | VfL Osnabrück | - | 1. FC Saarbrücken | 2:1 | ||||
| 22. Round | 22/01/2022 | SV Wehen Wiesbaden | - | VfL Osnabrück | 0:0 | ||||
| 23. Round | 25/01/2022 | VfL Osnabrück | - | Würzburger Kickers | 2:0 | ||||
| 30. Round | 13/03/2022 | FSV Zwickau | - | VfL Osnabrück | 1:3 | ||||
| 31. Round | 19/03/2022 | VfL Osnabrück | - | FC Viktoria 1889 Berlin | 3:1 | ||||
| 34. Round | 16/04/2022 | TSV 1860 München | - | VfL Osnabrück | 2:3 | ||||
| 32. Round | 19/04/2022 | Hallescher FC | - | VfL Osnabrück | 3:3 | ||||
| 35. Round | 23/04/2022 | VfL Osnabrück | - | Waldhof Mannheim | 1:2 | ||||
| 37. Round | 07/05/2022 | TSV Havelse | - | VfL Osnabrück | 0:1 |
Germany