| 18. Round | 15/03/2009 | Kickers Offenbach [Youth B] | - | 1. FSV Mainz 05 [Youth B] | 1:2 | ||||
| 20. Round | 05/04/2009 | 1. FC Kaiserslautern [Youth B] | - | 1. FSV Mainz 05 [Youth B] | 2:0 | ||||
| 22. Round | 25/04/2009 | 1. FC Nürnberg [Youth B] | - | 1. FSV Mainz 05 [Youth B] | 2:2 | ||||
| 23. Round | 17/05/2009 | 1. FSV Mainz 05 [Youth B] | - | Bayern München [Youth B] | 2:3 | ||||
| 24. Round | 24/05/2009 | VfB Stuttgart [Youth B] | - | 1. FSV Mainz 05 [Youth B] | 5:2 | ||||
| 25. Round | 07/06/2009 | 1. FSV Mainz 05 [Youth B] | - | SC Freiburg [Youth B] | 5:1 | ||||
| 26. Round | 14/06/2009 | SpVgg Greuther Fürth [Youth B] | - | 1. FSV Mainz 05 [Youth B] | 2:1 |
Germany