| 1. Round | 07/08/2009 | SSV Ulm 1846 | - | Bayern Alzenau | 4:1 | ||||
| 2. Round | 15/08/2009 | Bayern Alzenau | - | SG Sonnenhof Großaspach | 1:1 | ||||
| 3. Round | 22/08/2009 | SpVgg Greuther Fürth II | - | Bayern Alzenau | 3:2 | ||||
| 4. Round | 29/08/2009 | Bayern Alzenau | - | SC Pfullendorf | 0:1 | ||||
| 5. Round | 02/09/2009 | Karlsruher SC II | - | Bayern Alzenau | 0:1 |  |
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| 6. Round | 05/09/2009 | Bayern Alzenau | - | Eintracht Frankfurt II | 1:3 | ||||
| 7. Round | 12/09/2009 | 1. FC Eintracht Bamberg | - | Bayern Alzenau | 0:2 | |
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| 8. Round | 19/09/2009 | Bayern Alzenau | - | SC Freiburg II | 0:1 | |
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| 9. Round | 26/09/2009 | Bayern Alzenau | - | SSV Reutlingen | 1:3 | ||||
| 13. Round | 31/10/2009 | Bayern Alzenau | - | Stuttgarter Kickers | 1:3 | ||||
| 14. Round | 06/11/2009 | SV Wehen Wiesbaden II | - | Bayern Alzenau | 1:0 | ||||
| 15. Round | 21/11/2009 | Bayern Alzenau | - | SpVgg Weiden | 0:3 | |
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| 17. Round | 05/12/2009 | Bayern Alzenau | - | SV Darmstadt 98 | 1:0 | ||||
| 19. Round | 13/12/2009 | SG Sonnenhof Großaspach | - | Bayern Alzenau | 5:0 |
Germany