| 26. Round | 26/03/2010 | Eintracht Trier | - | Bayer Leverkusen II | 1:2 | ||||
| 24. Round | 30/03/2010 | SV 07 Elversberg | - | Bayer Leverkusen II | 1:0 |  |
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| 27. Round | 03/04/2010 | Bayer Leverkusen II | - | FC Schalke 04 II | 1:0 |  |
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| 28. Round | 10/04/2010 | Rot-Weiss Essen | - | Bayer Leverkusen II | 1:1 | |
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| 29. Round | 17/04/2010 | Bayer Leverkusen II | - | 1. FC Saarbrücken | 1:1 | ||||
| 31. Round | 02/05/2010 | Bayer Leverkusen II | - | SC Verl | 0:0 | ||||
| 32. Round | 07/05/2010 | Sportfreunde Lotte | - | Bayer Leverkusen II | 2:2 | ||||
| 33. Round | 22/05/2010 | Fortuna Düsseldorf II | - | Bayer Leverkusen II | 1:0 | ||||
| 34. Round | 29/05/2010 | Bayer Leverkusen II | - | Bor. Mönchengladbach II | 1:0 |
Germany