| 23. Round | 11/02/2001 | SG Wattenscheid 09 | - | 1. FC Union Berlin | 2:2 |  |
|||
| 24. Round | 17/02/2001 | 1. FC Union Berlin | - | Eintracht Braunschweig | 2:2 | |
|||
| 26. Round | 03/03/2001 | 1. FC Union Berlin | - | Sachsen Leipzig | 2:0 | |
 |
||
| 27. Round | 10/03/2001 | Erzgebirge Aue | - | 1. FC Union Berlin | 0:2 | |
|
||
| 28. Round | 18/03/2001 | 1. FC Union Berlin | - | Fortuna Köln | 2:0 | |
|||
| 29. Round | 31/03/2001 | SV Babelsberg 03 | - | 1. FC Union Berlin | 0:1 | |
|
||
| 30. Round | 07/04/2001 | 1. FC Union Berlin | - | Preußen Münster | 3:2 | |
|||
| 25. Round | 11/04/2001 | TeBe Berlin | - | 1. FC Union Berlin | 0:5 | |
|||
| 31. Round | 16/04/2001 | Fortuna Düsseldorf | - | 1. FC Union Berlin | 0:2 | |
|||
| 32. Round | 21/04/2001 | 1. FC Union Berlin | - | Werder Bremen II | 1:0 | |
|||
| 36. Round | 29/04/2001 | 1. FC Union Berlin | - | Rot-Weiss Essen | 1:0 | |
|||
| 33. Round | 05/05/2001 | Borussia Dortmund II | - | 1. FC Union Berlin | 0:2 | |
|||
| 34. Round | 12/05/2001 | 1. FC Union Berlin | - | SV Wilhelmshaven | 5:0 | |
|||
| 35. Round | 17/05/2001 | Dresdner SC | - | 1. FC Union Berlin | 0:0 | ||||
| 37. Round | 02/06/2001 | SC Verl | - | 1. FC Union Berlin | 2:1 | ||||
| 38. Round | 09/06/2001 | 1. FC Union Berlin | - | KFC Uerdingen 05 | 4:0 | |
Brazil