| 1. Round | 28/07/2024 | 1. FSV Mainz 05 II | - | Hessen Kassel | 3:0 | ||||
| 2. Round | 02/08/2024 | Eintracht Frankfurt II | - | 1. FSV Mainz 05 II | 0:0 |  |
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| 8. Round | 14/09/2024 | TSV Steinbach Haiger | - | 1. FSV Mainz 05 II | 2:1 | ||||
| 7. Round | 24/09/2024 | 1. FSV Mainz 05 II | - | Göppinger SV | 2:1 | ||||
| 10. Round | 28/09/2024 | FSV Frankfurt | - | 1. FSV Mainz 05 II | 1:0 | |
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| 11. Round | 05/10/2024 | 1. FSV Mainz 05 II | - | SGV Freiberg | 3:2 | ||||
| 13. Round | 20/10/2024 | 1. FSV Mainz 05 II | - | Bahlinger SC | 3:1 | ||||
| 12. Round | 23/10/2024 | 1899 Hoffenheim II | - | 1. FSV Mainz 05 II | 5:0 | |
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| 14. Round | 26/10/2024 | SG Barockstadt Fulda-Lehnerz | - | 1. FSV Mainz 05 II | 1:0 | ||||
| 18. Round | 23/11/2024 | Hessen Kassel | - | 1. FSV Mainz 05 II | 1:3 | ||||
| 19. Round | 30/11/2024 | 1. FSV Mainz 05 II | - | Eintracht Frankfurt II | 0:0 | ||||
| 20. Round | 08/12/2024 | FC 08 Homburg | - | 1. FSV Mainz 05 II | 4:0 | ||||
| 21. Round | 23/02/2025 | 1. FSV Mainz 05 II | - | FC 08 Villingen | 3:1 | |
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| 33. Round | 10/05/2025 | 1. FSV Mainz 05 II | - | Stuttgarter Kickers | 4:1 |
Germany