| 5. Round | 02/09/2009 | Karlsruher SC II | - | Bayern Alzenau | 0:1 | ||||
| 6. Round | 05/09/2009 | SSV Reutlingen | - | Karlsruher SC II | 0:1 | ||||
| 7. Round | 11/09/2009 | Karlsruher SC II | - | Hessen Kassel | 2:2 | ||||
| 8. Round | 18/09/2009 | VfR Aalen | - | Karlsruher SC II | 2:1 | ||||
| 9. Round | 26/09/2009 | Karlsruher SC II | - | 1. FC Nürnberg II | 0:2 | ||||
| 18. Round | 19/12/2009 | Karlsruher SC II | - | SC Pfullendorf | 3:2 | ||||
| 23. Round | 21/03/2010 | Karlsruher SC II | - | SSV Reutlingen | 3:0 | ||||
| 24. Round | 26/03/2010 | Hessen Kassel | - | Karlsruher SC II | 3:0 | ||||
| 26. Round | 13/04/2010 | 1. FC Nürnberg II | - | Karlsruher SC II | 3:0 | ||||
| 28. Round | 24/04/2010 | SV Wehen Wiesbaden II | - | Karlsruher SC II | 1:2 | ||||
| 29. Round | 30/04/2010 | Karlsruher SC II | - | SpVgg Weiden | 3:2 | ||||
| 31. Round | 12/05/2010 | Karlsruher SC II | - | SV Darmstadt 98 | 1:0 | ||||
| 32. Round | 15/05/2010 | SSV Ulm 1846 | - | Karlsruher SC II | 1:3 |  |
|||
| 33. Round | 22/05/2010 | Karlsruher SC II | - | SG Sonnenhof Großaspach | 3:2 |
Germany