| 2. Round | 14/08/2009 | 1. FC Magdeburg | - | Hamburger SV II | 0:0 | ||||
| 6. Round | 06/09/2009 | Hamburger SV II | - | VFC Plauen | 1:1 | ||||
| 8. Round | 19/09/2009 | Hamburger SV II | - | SV Babelsberg 03 | 1:6 | ||||
| 9. Round | 26/09/2009 | Hannover 96 II | - | Hamburger SV II | 0:2 | ||||
| 10. Round | 05/10/2009 | Hamburger SV II | - | Hallescher FC | 0:1 | ||||
| 13. Round | 31/10/2009 | Hertha BSC II | - | Hamburger SV II | 0:1 |  |
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| 15. Round | 22/11/2009 | VfL Wolfsburg II | - | Hamburger SV II | 0:0 | ||||
| 16. Round | 29/11/2009 | Hamburger SV II | - | FC Oberneuland | 2:0 | ||||
| 17. Round | 04/12/2009 | TeBe Berlin | - | Hamburger SV II | 1:4 | ||||
| 18. Round | 17/03/2010 | ZFC Meuselwitz | - | Hamburger SV II | 2:1 | |
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| 25. Round | 21/03/2010 | SV Babelsberg 03 | - | Hamburger SV II | 0:0 | ||||
| 19. Round | 24/03/2010 | Hamburger SV II | - | 1. FC Magdeburg | 1:1 | |
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| 26. Round | 27/03/2010 | Hamburger SV II | - | Hannover 96 II | 1:1 | ||||
| 33. Round | 22/05/2010 | FC Oberneuland | - | Hamburger SV II | 1:2 |
Germany