| 15. Round | 21/02/2010 | VfL Osnabrück [Youth B] | - | TeBe Berlin [Youth B] | 3:1 | ||||
| 16. Round | 27/02/2010 | Hannover 96 [Youth B] | - | VfL Osnabrück [Youth B] | 2:0 | ||||
| 18. Round | 20/03/2010 | Energie Cottbus [Youth B] | - | VfL Osnabrück [Youth B] | 0:2 | ||||
| 19. Round | 28/03/2010 | VfL Osnabrück [Youth B] | - | Werder Bremen [Youth B] | 2:2 | ||||
| 21. Round | 18/04/2010 | VfL Osnabrück [Youth B] | - | Hansa Rostock [Youth B] | 2:1 | ||||
| 22. Round | 24/04/2010 | FC Carl Zeiss Jena [Youth B] | - | VfL Osnabrück [Youth B] | 2:2 |  |
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| 14. Round | 02/05/2010 | Hertha BSC [Youth B] | - | VfL Osnabrück [Youth B] | 4:0 | ||||
| 23. Round | 08/05/2010 | VfL Osnabrück [Youth B] | - | 1. FC Union Berlin [Youth B] | 6:0 | |
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| 24. Round | 16/05/2010 | Holstein Kiel [Youth B] | - | VfL Osnabrück [Youth B] | 1:2 | |
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| 17. Round | 25/05/2010 | VfL Osnabrück [Youth B] | - | VfL Wolfsburg [Youth B] | 0:1 | ||||
| 25. Round | 06/06/2010 | VfL Osnabrück [Youth B] | - | Hamburger SV [Youth B] | 2:1 | |
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| 26. Round | 13/06/2010 | Rot-Weiß Erfurt [Youth B] | - | VfL Osnabrück [Youth B] | 0:3 | |
Germany