| 1. Round | 04/09/1949 | Rot-Weiß Oberhausen | - | Duisburger SpV | 1:1 | ||||
| 2. Round | 11/09/1949 | Duisburger SpV | - | Preußen Dellbrück | 2:4 | ||||
| 8. Round | 23/10/1949 | Duisburger SpV | - | TSG Vohwinkel 80 | 4:0 | ||||
| 9. Round | 30/10/1949 | 1. FC Köln | - | Duisburger SpV | 2:3 | ||||
| 10. Round | 06/11/1949 | Duisburger SpV | - | Arminia Bielefeld | 2:0 | ||||
| 11. Round | 13/11/1949 | Duisburger SpV | - | SF Hamborn 07 | 2:2 | ||||
| 12. Round | 20/11/1949 | FC Schalke 04 | - | Duisburger SpV | 4:1 | ||||
| 14. Round | 18/12/1949 | Alemannia Aachen | - | Duisburger SpV | 3:3 | ||||
| 3. Round | 08/01/1950 | STV Horst-Emscher | - | Duisburger SpV | 8:2 | ||||
| 16. Round | 15/01/1950 | Preußen Dellbrück | - | Duisburger SpV | 2:0 | ||||
| 17. Round | 22/01/1950 | Duisburger SpV | - | STV Horst-Emscher | 1:1 | ||||
| 18. Round | 29/01/1950 | Borussia Dortmund | - | Duisburger SpV | 7:0 | ||||
| 19. Round | 05/02/1950 | Duisburger SpV | - | Rot-Weiß Oberhausen | 3:2 | ||||
| 20. Round | 12/02/1950 | Duisburger SpV | - | SV Rhenania Würselen | 0:1 | ||||
| 22. Round | 05/03/1950 | TSG Vohwinkel 80 | - | Duisburger SpV | 0:2 | ||||
| 23. Round | 12/03/1950 | Duisburger SpV | - | Duisburger FV 08 | 2:1 | ||||
| 24. Round | 19/03/1950 | Duisburger SpV | - | 1. FC Köln | 1:1 |
Germany